REPORT OF THE 23rd UCLA INTERNATIONAL KIR EXCHANGE

For the 23rd KIR Exchange, 4 DNA samples (KDNA#89-92) were shipped to each laboratory on August 4, 2010. Sixty-one laboratories submitted their KIR typing results. The majority of the laboratories used either commercial or in-house developed sequence-specific primer (SSP) based PCR typing systems, and others used sequence-specific oligonucleotide probing (SSOP) method. The results for the 23rd KIR Exchange are summarized in Table 1 and individual laboratory results reported for each DNA sample are provided in Tables 2-5. Subtyping results for KIR2DL2, 2DL5, 3DL1, 3DS1, 2DS1, 2DS4, and/or 3DP1 loci were reported by some laboratories. The positive/negative assignments for all 4 DNA samples were reported without any discrepancy for 10 of the 16 tested KIR loci (KIR2DL1, 2DL3, 2DL4, 2DL5, 3DL2, 3DL3, 3DS1, 2DS1, 2DP1, and 3DP1). Discrepant results from the consensus typing are italicized in tables 1-5, and described in the summation for each sample. Discrepancies at the allele level are not italicized. We encourage the participating laboratories to resolve the discrepancies so that the information can be shared to develop reliable KIR typing systems. We thank you for your continued participation in the UCLA KIR Exchange program.